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biotinylated recombinant human rh il10 antigens  (R&D Systems)


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    Structured Review

    R&D Systems biotinylated recombinant human rh il10 antigens
    Antigen-antibody recognition mechanism and biosensing experiments. ( a ) Positive test: target <t>biotinylated</t> <t>IL10</t> antigens in solution recognize and bind to the probe antibodies anchored to the surface; fluorescent avidin binds to the biotin, and it is detected by fluorescence microscopy; ( b ) negative test: biotinylated not-matching antigens do not bind with the probe antibodies, and they are washed away, no fluorescence signal can be detected. ( c ) Epifluorescence microscopy images of the funnel after a positive test performed with a biotinylated IL10 concentration of 1.2 pg/mL (right) and after a negative test (left).
    Biotinylated Recombinant Human Rh Il10 Antigens, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated recombinant human rh il10 antigens/product/R&D Systems
    Average 92 stars, based on 36 article reviews
    biotinylated recombinant human rh il10 antigens - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Nanofluidic-Based Accumulation of Antigens for Miniaturized Immunoassay"

    Article Title: Nanofluidic-Based Accumulation of Antigens for Miniaturized Immunoassay

    Journal: Sensors (Basel, Switzerland)

    doi: 10.3390/s20061615

    Antigen-antibody recognition mechanism and biosensing experiments. ( a ) Positive test: target biotinylated IL10 antigens in solution recognize and bind to the probe antibodies anchored to the surface; fluorescent avidin binds to the biotin, and it is detected by fluorescence microscopy; ( b ) negative test: biotinylated not-matching antigens do not bind with the probe antibodies, and they are washed away, no fluorescence signal can be detected. ( c ) Epifluorescence microscopy images of the funnel after a positive test performed with a biotinylated IL10 concentration of 1.2 pg/mL (right) and after a negative test (left).
    Figure Legend Snippet: Antigen-antibody recognition mechanism and biosensing experiments. ( a ) Positive test: target biotinylated IL10 antigens in solution recognize and bind to the probe antibodies anchored to the surface; fluorescent avidin binds to the biotin, and it is detected by fluorescence microscopy; ( b ) negative test: biotinylated not-matching antigens do not bind with the probe antibodies, and they are washed away, no fluorescence signal can be detected. ( c ) Epifluorescence microscopy images of the funnel after a positive test performed with a biotinylated IL10 concentration of 1.2 pg/mL (right) and after a negative test (left).

    Techniques Used: Avidin-Biotin Assay, Fluorescence, Microscopy, Epifluorescence Microscopy, Concentration Assay



    Similar Products

    biotinylated recombinant human rh il10 antigens  (R&D Systems)
    92
    R&D Systems biotinylated recombinant human rh il10 antigens
    Antigen-antibody recognition mechanism and biosensing experiments. ( a ) Positive test: target <t>biotinylated</t> <t>IL10</t> antigens in solution recognize and bind to the probe antibodies anchored to the surface; fluorescent avidin binds to the biotin, and it is detected by fluorescence microscopy; ( b ) negative test: biotinylated not-matching antigens do not bind with the probe antibodies, and they are washed away, no fluorescence signal can be detected. ( c ) Epifluorescence microscopy images of the funnel after a positive test performed with a biotinylated IL10 concentration of 1.2 pg/mL (right) and after a negative test (left).
    Biotinylated Recombinant Human Rh Il10 Antigens, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated recombinant human rh il10 antigens/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    biotinylated recombinant human rh il10 antigens - by Bioz Stars, 2026-03
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Antigen-antibody recognition mechanism and biosensing experiments. ( a ) Positive test: target biotinylated IL10 antigens in solution recognize and bind to the probe antibodies anchored to the surface; fluorescent avidin binds to the biotin, and it is detected by fluorescence microscopy; ( b ) negative test: biotinylated not-matching antigens do not bind with the probe antibodies, and they are washed away, no fluorescence signal can be detected. ( c ) Epifluorescence microscopy images of the funnel after a positive test performed with a biotinylated IL10 concentration of 1.2 pg/mL (right) and after a negative test (left).

    Journal: Sensors (Basel, Switzerland)

    Article Title: Nanofluidic-Based Accumulation of Antigens for Miniaturized Immunoassay

    doi: 10.3390/s20061615

    Figure Lengend Snippet: Antigen-antibody recognition mechanism and biosensing experiments. ( a ) Positive test: target biotinylated IL10 antigens in solution recognize and bind to the probe antibodies anchored to the surface; fluorescent avidin binds to the biotin, and it is detected by fluorescence microscopy; ( b ) negative test: biotinylated not-matching antigens do not bind with the probe antibodies, and they are washed away, no fluorescence signal can be detected. ( c ) Epifluorescence microscopy images of the funnel after a positive test performed with a biotinylated IL10 concentration of 1.2 pg/mL (right) and after a negative test (left).

    Article Snippet: In particular, we prepared solutions of matching antigens consisting of biotinylated recombinant human (rh) IL10 antigens (NF100 kit Fluorokine ® Biotinylated Human IL10, R&D system) diluted in PBS 1X at different concentrations (1.2 pg/mL, 12 pg/mL and 120 pg/mL) and of not-matching antigens, i.e., the negative control reagent provided by the NF100 kit (the soybean trypsin inhibitor biotinylated to the same degree as the cytokine).

    Techniques: Avidin-Biotin Assay, Fluorescence, Microscopy, Epifluorescence Microscopy, Concentration Assay